The Effect of 8 Weeks of Endurance Training with Stevia Supplementation on the Expression of Genes (POMC and MC4R) Involved in Hypothalamic Axis Metabolism in Obese Male Wistar Rats

Mohammadi, Farzaneh; Younesian, Ali; Zia-ul-Haq, Seyed Javad

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Mohammadi F, Younesian A, Zia-ul-Haq S J. The Effect of 8 Weeks of Endurance Training with Stevia Supplementation on the Expression of Genes (POMC and MC4R) Involved in Hypothalamic Axis Metabolism in Obese Male Wistar Rats. MEJDS 2023; 13 :8-8
URL: http://jdisabilstud.org/article-1-2753-en.html

The Effect of 8 Weeks of Endurance Training with Stevia Supplementation on the Expression of Genes (POMC and MC4R) Involved in Hypothalamic Axis Metabolism in Obese Male Wistar Rats

Farzaneh Mohammadi¹

, Ali Younesian ^*²

, Seyed Javad Zia-ul-Haq³

1- MSc Student, Faculty of Physical Education and Sport Sciences, Sports Physiology, Shahroud University of Technology, Shahroud, Iran
2- Associate Professor, Faculty of Physical Education and Sport Sciences, Sports Physiology, Shahroud University of Technology, Shahroud, Iran
3- Assistant Professor, Department of Sports Physiology, Shahroud Branch, Islamic Azad University, Shahroud, Iran

Abstract: (846 Views)

Abstract
Background & Objectives: Obesity results from complex interactions of environment, genetic factors, and human behavior. Studies of families, adopted children, and twins have shown that 45%–75% of changes in body mass index (BMI) between individuals are due to genetic factors. The melanocortin 4 receptor (MC4R) gene is a single exon and expresses a protein receptor containing 323 amino acids. Mutations in this gene cause partial or complete loss of function of this receptor, and by disrupting the energy homeostasis system, it is the most common cause of monogenic obesity and an important component of polygenic obesity. Studies on the effect of exercise and dietary supplements on the expression of hypothalamic genes involved in appetite and obesity are limited. Therefore, this study investigated the effect of 8 weeks of endurance training with stevia supplementation on the expression of genes (POMC and MC4R) involved in hypothalamic axis metabolism in obese male Wistar rats.
Methods: In the present experimental study, 25 adult Wistar rats were divided into 5 groups. Four groups underwent a high–calorie diet to gain weight; one group followed a normal diet. The groups were as follows: exercise–obese, stevia supplement–obese, exercise and stevia supplement–obese, obese, and control. The supplement groups received stevia at a dose of 250 mg/kg body weight of rats 5 days a week for 8 weeks. The training program consisted of walking on a treadmill with an intensity of 50% to 70% oxygen consumption, which started at 15 m/min in the first week and reached a speed of 22 m/min in the eighth week. Also, in the first and last 5 minutes, walking at a speed of 10 m/min was performed to warm up and cool down, respectively. Finally, the mice were anesthetized using a combination of 70 to 30 ketamine and xylazine (the injection rate was one large syringe/100 g body weight). Tissue sampling was performed in under 90 s. Tissue was cast into microtube shield RNA after selection. Immediately after the mRNAs were fixed, they were transferred into liquid nitrogen at –180°C and sent to the laboratory. It was placed in the laboratory at –80°C, and the genes were extracted. Data were analyzed using 1–way analysis of variance (ANOVA) and LSD post hoc test by SPSS software version 19 at a significance level of 0.05.
Results: The ANOVA showed no significant difference between the studied groups in the MC4R gene (p=0.684). But a significant difference was observed in the POMC gene (p=0.031). Based on the results of the LSD post hoc test, there was a significant difference in POMC gene expression between the obese group and the control group (p=0.003) and between the obese group and the stevia plus exercise group (p=0.015).
Conclusion: Based on the findings of this study, endurance training and stevia supplementation increased the expression of genes (POMC and MC4R) involved in hypothalamic axis metabolism and subsequently decreased appetite.

Keywords: MC4R, POMC, Stevia, Endurance training

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Type of Study: Original Research Article | Subject: Rehabilitation

References

1. Blüher M. Obesity: global epidemiology and pathogenesis. Nat Rev Endocrinol. 2019;15(5):288–98. [DOI]

2. World Health Organization. Obesity and overweight [Internet]; 2021. [Article]

3. Hruby A, Hu FB. The epidemiology of obesity: a big picture. Pharmacoeconomics. 2015;33(7):673–89. [DOI]

4. Djalalinia S, Saeedi Moghaddam S, Sheidaei A, Rezaei N, Naghibi Iravani SS, Modirian M, et al. Patterns of obesity and overweight in the Iranian population: findings of STEPs 2016. Front Endocrinol (Lausanne). 2020;11:42. [DOI]

5. Cheung WW, Mao P. Recent advances in obesity: genetics and beyond. ISRN Endocrinol. 2012;2012:536905. [DOI]

6. Pérusse L, Chagnon YC, Weisnagel SJ, Rankinen T, Snyder E, Sands J, et al. The human obesity gene map: the 2000 update. Obes Res. 2001;9(2):135–69. [DOI]

7. Tao YX. The melanocortin–4 receptor: Physiology, pharmacology, and pathophysiology. Endocr Rev. 2010;31(4):506–43. [DOI]

8. Beckers S, Zegers D, Van Gaal LF, Van Hul W. The role of the leptin–melanocortin signalling pathway in the control of food intake. Crit Rev Eukaryot Gene Expr. 2009;19(4):267–87. [DOI]

9. Govaerts C, Srinivasan S, Shapiro A, Zhang S, Picard F, Clement K, et al. Obesity–associated mutations in the melanocortin 4 receptor provide novel insights into its function. Peptides. 2005;26(10):1909–19. [DOI]

10. Valette M, Bellisle F, Carette C, Poitou C, Dubern B, Paradis G, et al. Eating behaviour in obese patients with melanocortin–4 receptor mutations: a literature review. Int J Obes (Lond). 2013;37(8):1027–35. [DOI]

11. Monazamnezhad A, Habibi A, Shakeriyan S, Majdinasab N, Ghalvand A. The effects of aerobic exercise on lipid profile and body composition in women with multiple sclerosis. Jundishapur J Chronic Dis Care. 2015;4(1); e26619. [DOI]

12. Gadde KM, Allison DB, Ryan DH, Peterson CA, Troupin B, Schwiers ML, et al. Effects of low–dose, controlled-release, phentermine plus topiramate combination on weight and associated comorbidities in overweight and obese adults (CONQUER): a randomised, placebo-controlled, phase 3 trial. Lancet. 2011;377(9774):1341–52. [DOI]

13. Smith SR, Weissman NJ, Anderson CM, Sanchez M, Chuang E, Stubbe S, et al. Multicenter, placebo–controlled trial of lorcaserin for weight management. N Engl J Med. 2010;363(3):245–56. [DOI]

14. Jeong IY, Lee HJ, Jin CH, Park YD, Choi DS, Kang MA. Anti–inflammatory activity of stevia rebaudiana in LPS–induced RAW 264.7 cells. Journal of Food Science & Nutrition. 2010;15(1):14–8. [DOI]

15. Silva GEC da, Assef AH, Albino CC, Ferri L de AF, Tasin G, Takahashi MH, et al. Investigation of the tolerability of oral stevioside in Brazilian hyperlipidemic patients. Brazilian Archives of Biology and Technology. 2006;49:583–7. [DOI]

16. Assaei R, Mokarram P, Dastghaib S, Darbandi S, Darbandi M, Zal F, et al. Hypoglycemic effect of aquatic extract of stevia in pancreas of diabetic rats: PPARγ–dependent regulation or antioxidant potential. Avicenna J Med Biotechnol. 2016;8(2):65–74.

17. Akbarzadeh S, Eskandari F, Tangestani H, Bagherinejad ST, Bargahi A, Bazzi P, et al. The effect of Stevia rebaudiana on serum omentin and visfatin level in STZ-induced diabetic rats. J Diet Suppl. 2015;12(1):11–22. [DOI]

18. Asghari G, Mirmiran P, Rashidkhani B, Asghari-Jafarabadi M, Mehran M, Azizi F. The association between diet quality indices and obesity: Tehran lipid and glucose study. Arch Iran Med. 2012;15(10):599–605.

19. Casazza K, Dulin–Keita A, Gower BA, Fernandez JR. Differential influence of diet and physical activity on components of metabolic syndrome in a multiethnic sample of children. J Am Diet Assoc. 2009;109(2):236–44. [DOI]

20. Hemati Nafar M, Kordi MR, Chubineh S, Chubineh S. The effect of six-weeks high intensity interval training (HIIT) on fibrinolytic factors (t-PA, PAI-1& t-PA/PAI-1) in sedentary young men. Journal of Sport Biosciences. 2013;5(3):77–89. [Persian] [DOI]

21. Shi X, Zhou X, Chu X, Wang J, Xie B, Ge J, et al. Allicin improves metabolism in high–fat diet–induced obese mice by modulating the gut microbiota. Nutrients. 2019;11(12):2909. [DOI]

22. Lee MH, Kim H, Kim SS, Lee TH, Lim BV, Chang HK, et al. Treadmill exercise suppresses ischemia-induced increment in apoptosis and cell proliferation in hippocampal dentate gyrus of gerbils. Life Sci. 2003;73(19):2455–65. [DOI]

23. Benite–Ribeiro SA, Putt DA, Santos JM. The effect of physical exercise on orexigenic and anorexigenic peptides and its role on long–term feeding control. Med Hypotheses. 2016;93:30–3. [DOI]

24. Caruso V, Bahari H, Morris MJ. The beneficial effects of early short–term exercise in the offspring of obese mothers are accompanied by alterations in the hypothalamic gene expression of appetite regulators and FTO (fat mass and obesity associated) gene. J Neuroendocrinol. 2013;25(8):742–52. [DOI]

25. Jiaxu C, Weiyi Y. Influence of acute and chronic treadmill exercise on rat brain POMC gene expression. Med Sci Sports Exerc. 2000;32(5):954–7. [DOI]

26. Lorenzeti FM, Fojo D, Chaves S. Does resistance training modulate food intake and hypothalamic neuropepetides mRNA expression in rats. Clinical and Experimental Medical Sciences. 2013;1(7):299–308.

27. Khazali H, Khajehnasiri N, Sheikhzadeh Hesari F. Alterations of hypothalamic pro–opiomelanocortin gene expression and appetite in response, to one–month regular moderate exercise. Experimental animal Biology. 2018;7(2):125–34. [Persian] [DOI]

28. Cintra DE, Ropelle ER, Pauli JR. Brain regulation of food intake and expenditure energy: molecular action of insulin, leptin and physical exercise. Rev Neurol. 2007;45(11):672–82. [Spanish]

29. Benite-Ribeiro SA, Santos JMD, Duarte JAR. Moderate physical exercise attenuates the alterations of feeding behaviour induced by social stress in female rats. Cell Biochem Funct. 2014;32(2):142–9. [DOI]